Born in Trenton, New Jersey, Dr. James Miele attended Delaware Valley College and graduated with a Bachelor of Science in Animal Science in 1977. Following his undergraduate studies, he attended Rutgers University and Ohio State University, where he received Master's Degrees in Animal Science and Pathology. In 1982, Dr. Miele was admitted to Ohio State University's School of Veterinary Medicine and was awarded his Doctorate of Veterinary Medicine in 1986.

A general veterinary practitioner at Princeton Animal Hospital, Dr. Miele's area of particular professional interest is in surgery. He and his wife, Dr. Terry Miele, co-own the Hospital, which they purchased in 1988.

Dr. Miele and his wife have 5 children named Theresa, Elizabeth, Emily, Sam, and Greg. The children's ages range from 15 to 28. During his leisure time, Dr. Miele enjoys training for triathlons, reading non-fiction and is involved with his son in scouting.